Figure 3

(a) Pharmacologic demethylation and reactivation of WIF1 with 5-aza-2′-deoxycytidine (Aza) treatment in NPC and ESCC cells. Semiquantitative RT-PCR analysis detected upregulated expression and MSP detected concomitant demethylation of the promoter in Aza-treated cell lines. (b) Detailed BGS analysis showed that the percentage of methylated CpG sites was significantly reduced after treatment with Aza.