Figure 2 | Laboratory Investigation

Figure 2

From: Interaction of Toll-like receptors with bacterial components induces expression of CDX2 and MUC2 in rat biliary epithelium in vivo and in culture

Figure 2

Expression of MUC2 or CDX2 protein and mRNA level at intestinal metaplasia of the intrahepatic bile ducts of PCK rats at 10 months. (a–c) Immunohistochemistry for MUC2 (a) and CDX2 (b), and double fluorescence immunohistochemistry for MUC2 and CDX2 (c). (a) MUC2 is expressed in the cytoplasmic mucin of goblet cells (arrow) and also secreted in the lumen. Counterstained with hematoxylin, × 400. (b) CDX2 is expressed in the nuclei of goblet cells (arrow) and of surrounding columnar BECs (arrowhead). Counterstained with hematoxylin, × 400. (c) Double fluorescence immunostaining for MUC2 (green) and CDX2 (red). In goblet cells, MUC2 in the cytoplasm and CDX2 in the nucleus are coexpressed. In nuclei of some columnar BECs around goblet cells, CDX2 is expressed, whereas MUC2 expression is not evident. *Lumen of the bile duct. × 400. (d–g) ISH for MUC2 mRNA with antisense (d) and sense (e) probes and for CDX2 with antisense (f) and sense (g) probes. Positive staining of MUC2 and CDX2 mRNA is evident in the cytoplasm of goblet cells (d and f, arrows). The signals of CDX2 mRNA are also seen in the cytoplasm of columnar BECs around goblet cells (f, arrowheads). These signals were not detected when the slides were hybridized with sense probes (e and g). (d–g) Counterstained with methyl green, × 400.

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