Figure 3 | Laboratory Investigation

Figure 3

From: Interaction of Toll-like receptors with bacterial components induces expression of CDX2 and MUC2 in rat biliary epithelium in vivo and in culture

Figure 3

Western blot analysis for CDX2 protein in rat liver tissue. (a) Positive signals for CDX2 protein (40 kDa) are detected in the liver of PCK rats showing chronic suppurative cholangitis. Expression of CDX2 is stronger in the liver tissues that show moderate to severe cholangitis and intestinal metaplasia (10 months (M) and 16M), whereas CDX2 is weakly detected in PCK rat with mild cholangitis but no intestinal metaplasia (10M). CDX2 protein is not detected in samples of PCK rat with no cholangitis or intestinal metaplasia and also the control rat. Expression of β-actin (42 kDa) was detected in all samples. (b) CDX2 is detectable in the biliary tree freshly isolated from the liver of PCK rat showing cholangitis and intestinal metaplasia at 8 months (right lane). Instead, CDX2 protein is not detectable in the cultured BECs freshly isolated from the liver of PCK rat at 8 weeks without cholangitis (left lane).

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