Table 2 Antibodies and commercially purchased reagents used in this study
Source | Type of antibody | Animals immunized | Specificity of antibody | Optimal dilution/ concentration | |
|---|---|---|---|---|---|
Anti-MUC2a | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | Poly | Rabbit (IgG) | Mouse, rat, and human | 1:100 |
Anti-MUC2b | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | Poly | Goat (IgG) | Mouse, rat, and human | 1:100 |
Anti-CDX2a,b | BioGenex (SanRamon, CA, USA) | Mono | Mouse (IgG) | Human | 1:100 |
Anti-TLR2a,c | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | Poly | Rabbit (IgG) | Mouse, rat, and human | 1:100 |
Anti-TLR4a,c | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | Poly | Rabbit (IgG) | Human, mouse, and rat | 1:100 |
Anti-β-actinb | Abcam (Cambridge, UK) | Mono | Mouse (IgG) | Various species | 1:5000 |
Envision+ | DAKO (Tokyo, Japan) | ||||
DAPI | Molecular Probes (Eugene, OR, USA) | 1:500 | |||
SP6/7 RNA polymerase | Promega Corporation (Madison, WI, USA) | ||||
T-PER | Pierce (Rockkford, IL, USA) | ||||
RNeasy total RNA system | Qiagen (Hilden, Germany) | ||||
LPS | Sigma (from Escherichia coli serotype o55:B5) | 0.1–10 μg/ml | |||
LTA | Sigma (from Streptococcus faecalis) | 100 μg/ml | |||
PGN | Sigma (from Staphylococcus aureus) | 100 μg/ml | |||
Pam3 | EMC microcollections (Tubingen, Germany) | 0.1–10 μg/ml | |||
MG132 | Calbiochem (San Diego, CA, USA) | 1 μM | |||
siRNA | Qiagen (Hilden, Germany) | ||||
DreamFect™ | OZ Bioscience (Marseilles, France) | ||||
OPTI-MEMI | Life Technologies (Rockville, MD, USA) | ||||
FuGENE 6 | Roche Diagnostics (Indianapolis, IN, USA) |
