Figure 1 | Laboratory Investigation

Figure 1

From: Androgens influence expression of matrix proteins and proteolytic factors during cutaneous wound healing

Figure 1

Wound collagen accumulation is increased as a consequence of castration. (a) Day 6 wound tissue from intact (IN) and castrated (CSX) rats was analysed for collagen content by Picro-Sirius staining (top panels) and immunohistochemical expression of collagen I (middle panels) and III (bottom panels). (a, b) Overall day 6 wound type I collagen (but not collagen III) levels were increased in castrated animals. (c) Expression of the genes encoding the type I and III collagen polypeptides was not significantly affected by castration (data expressed as mean±s.e.m.). Rat dermal fibroblast production of type I collagen (d) was increased as a result of treatment with DHT or testosterone (T). n=5–6 per treatment group. *P<0.05 (intact vs CSX). Scale bars define a distance of 100 μM (Picro-Sirius red staining) or 25 μM (collagen immunostaining).

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