Figure 2
Wound fibronectin levels are increased in castrated rats. (a) Day 6 wound tissue from intact (IN) and castrated (CSX) rats was analysed for secreted (upper panels) and cellular fibronectin (FN) content (lower panels) by immunohistochemistry. (a, b) While numbers of fibronectin-immunoreactive cells in day 6 wounds were unaffected by castration, overall day 6 wound fibronectin levels were increased. (c) Expression of the Fn1 gene, which encodes fibronectin was not significantly affected by castration. (d) Rat dermal fibroblast fibronectin production was unaffected by treatment with DHT or testosterone (T). n=5–6 per treatment group. Cell count and mRNA expression data are expressed as mean±s.e.m. *P<0.05 (intact vs CSX). Scale bars define a distance of 25 μM. Arrows identify positively stained cells.
