Figure 5
Liver regeneration after CCl4 injection. (a) Liver section. HE stain shows hepatocyte necrosis (brighter red area) in L-Stat3−/− and littermate Stat3f/+ controls 40 h after CCl4 injection. (b) Summary of necrotic area. There was no significant difference between the area of necrosis in L-Stat3−/− (n=6, black bar) and Stat3f/+ mice (n=6, white bar) 40 h after CCl4 injection. (c) Summary of BrdU-positive hepatocytes at 40 h after CCl4 injection. BrdU-labeled hepatocytes were counted 24, 40, 48 and 72 h after CCl4 injection. Four to six mice were used for each of the L-Stat3−/− and Stat3f/+ group at each time point. *P<0.01 between L-Stat3−/− and Stat3f/+ mice. (d) Immunohistochemical staining of BrdU (red) in liver sections at 40 h after CCl4 injection. Nuclei were counter stained with DAPI (blue). Original magnification: × 40.
