Figure 6
STAT1 activation after hepatectomy and inflammatory infiltration after CCl4 injection. (a) STAT1 and STAT3 DNA binding activity in post hepatectomy liver. Nuclear extracts from liver tissue were obtained at different time points after PH, incubated with radiolabelled m67-SIE oligonucleotides and analyzed by EMSA. Position of STAT3 and STAT1 is indicated by arrows. STAT1 activation is detectable after hepatectomy in KO mice. STAT1: STAT1 homodimer; STAT1/STAT3: STAT1 and STAT3 heterodimer; STAT3: STAT3 homodimer; 3 h sham: liver nuclear extracts from mice 3 h after sham operation. (b) Histopathological examination of liver sections after CCl4 injection. Microphotos: HE staining of liver sections from L-Stat3−/− (n=6) and Stat3f/+ mice (n=6) was performed 40 h after CCl4 injection. For each mouse, 30 sections were made and the microphotos show representative foci of L-Stat3−/− and Stat3f/+ mice. The magnified view shows neutrophil (arrows) and monocyte (arrow heads) infiltration in the liver of L-Stat3−/− mice. Lower right panel: for each mouse, 10 randomized, blinded sections were used to count neutrophils and monocytes according to the distinct morphology under high power magnification (open bars: Stat3f/+, and closed bars: L-Stat3−/− mice). Original magnification: × 40. *P<0.01 between L-Stat3−/− and Stat3f/+.
