Figure 2
From: IL-33 can promote survival, adhesion and cytokine production in human mast cells
rhIL-1β, rhIL-18 or rhIL-33 induce phosphorylation of MAP kinases in naïve HUCBMCs. Naïve HUCBMCs were cultured with 100 ng/ml of rhIL-1β, rhIL-18 or rhIL-33 for the indicated time periods. Whole-cell lysates were then prepared and the phosphorylation of MAP kinases was assessed by Western blot analysis. The data are representative of the similar results that were obtained in independent experiments conducted using at least two distinct batches of HUCBMCs for analysis of each cytokine.
