Figure 1 | Laboratory Investigation

Figure 1

From: EMT tumorigenesis in the mouse mammary gland

Figure 1

Met-1 and Met-1 AS-OPN tumor cohorts. Immunohistochemical analyses of CK8/18 (a, b), CK14 (c, d), smooth muscle actin (SMA) (e, f), vimentin (g, h), E-cadherin (i, j) and PyVmT (k, l) of tumors derived from Met-1 (a, c, e, g, i, k) and Met-1 AS-OPN (b, d, f, h, j, l) cells. Met-1 tumors show an epithelial pattern characteristic of adenocarcinomas with glandular structures and polygonal cells, which are positive for CK8/18 (a) and E-cadherin (i). CK14 (c) and SMA (e) are present only surrounding the glandular structures and vimentin is expressed only in the stromal cells (g). In contrast, Met-1 AS-OPN tumors present morphological characteristics of spindle cells, which are positive only for mesenchymal markers such as SMA (f) and vimentin (h). The Met-1 AS-OPN tumors also show loss of expression of E-cadherin (j) and of the oncogene PyVmT (l). All images (a–l) have identical magnifications with a 50 μm scale bar shown in (l).

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