Figure 2 | Laboratory Investigation

Figure 2

From: Inhibition of proteasome by bortezomib causes intracellular aggregation of hepatic serpins and increases the latent circulating form of antithrombin

Figure 2

Effects of bortezomib on human hepatoma HepG2 cells after 24 h of incubation. (a) Secretion of antithrombin from HepG2 cells into the tissue culture medium. Results are presented as means±s.e.m. of five different experiments (***P<0.001). (b) Dose-dependent accumulation of intracellular antithrombin, as detected by SDS-PAGE and immunoblotting. (c) Dose-dependent effects of the drug on intracellular antithrombin and α1-antitrypsin as revealed by indirect immunofluorescence. Arrows indicate intracellular accumuli of antithrombin (AT) or α1-antitrypsin (α1-AT).

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