Figure 4

Time-dependent histological and ultrastructural effects of bortezomib on mice livers. (a) Haematoxylin and eosin ( × 40 magnification), congo red (CR) (featuring β-amyloid deposit staining) and amylase-PAS (PAS) (featuring glycoprotein accumuli) ( × 100 magnification in both cases) under light microscopy. Additionally, transmission electron microscopy (EM) revealed ultrastructural features of bortezomib-treated hepatocytes from mice ( × 8000 magnification). Black arrows indicate the presence of intracellular Mallory bodies and/or glycoprotein deposits. Red arrows indicate areas of dilated ER cisternae. (b) Digital amplification of confronting cisternae and dilated ER from a mouse treated with bortezomib for 4 days under electron microscopy. Red arrows indicate areas of dilated ER cisternae.