Figure 2
From: The aspartic protease napsin A suppresses tumor growth independent of its catalytic activity
(a) Structures of wild-type and mutant napsin A cDNA constructs. The first amino acid (Met) predicted from the full cDNA sequence is numbered as 1. Deduction of the initial residue of mature protein is based on the result by Schauer-Vukasinovic et al.25 In napsinD283N, Asp283 in the catalytic site was changed to an Asn by site-directed mutagenesis. Napsin cDNA constructs have HA tags in their C-termini. (b) Protein expression levels of each clone. Clones that express napsin A or the catalytically inactive napsinD283N protein were analyzed together with control clones transfected with empty vector. The expression level is shown by western blotting using an anti-HA antibody with tubulin as control.
