Figure 5
From: The aspartic protease napsin A suppresses tumor growth independent of its catalytic activity
Inhibition of anchorage-independent growth of HEK293 cells by napsin A. Five hundred cells of each clone were suspended in 0.35% soft agar in three replica wells of 12-well plates. After 10 days, colonies with more than 20 cells were counted. All three clones stably transfected with napsin A formed fewer colonies in soft agar than the control cells transfected with vector alone. Both clones expressing napsinD283N formed soft agar colonies with a similar efficiency as cells expressing wild-type napsin A.
