Figure 1
From: SHP-1 deficiency and increased inflammatory gene expression in PBMCs of multiple sclerosis patients
SHP-1 mRNA levels in freshly isolated PBMCs of MS patients and normal subjects. The absolute mRNA transcript copy numbers per 10 ng of total RNA were quantified using real-time RT-PCR in MS patients (n=23) and normal subjects (n=18). (a) Using primers in the mid-region of the SHP-1 gene that would amplify both SHP-1 transcripts, the levels of the common SHP-1 transcripts were measured (*significance of P<0.05). (b) The mRNA levels of the housekeeping gene GAPDH were quantified between MS patients and normal subjects. (c) Sequence-specific primers to the SHP-1 gene were used to quantify the expression of the SHP-1 promoter I transcripts. (d) Similarly, sequence-specific primers allowed the quantification of the SHP-1 promoter II transcripts.
