Figure 4
From: SHP-1 deficiency and increased inflammatory gene expression in PBMCs of multiple sclerosis patients
SHP-1 mRNA levels in freshly isolated (a–d) or cultured (e–h) PBMCs of patients diagnosed with different clinical subclassifications of MS. The absolute specific mRNA transcript numbers per 10 ng of total RNA were quantified with real-time RT-PCR in patients with active relapsing–remitting (RR) MS (n=10), active secondary progressive (SP) MS (n=6), or inactive RR (Inc) MS (n=7). The mRNA levels of the (a) common SHP-1 transcript, (b) housekeeping gene GAPDH, (c) SHP-1 promoter I transcripts, and (d) SHP-1 promoter II transcripts were quantified in freshly isolated PBMCs of MS patients. Similarly, the mRNA levels of (e) the common SHP-1 transcript, (f) the housekeeping gene GAPDH, (g) SHP-1 promoter I transcripts, and (h) SHP-1 promoter II transcripts were quantified in PBMCs of MS patients that were cultured for 1 week.
