Figure 5
From: SHP-1 deficiency and increased inflammatory gene expression in PBMCs of multiple sclerosis patients
Quantification of the SHP-1 mRNA in different cell types of MS patients and normal subjects. The absolute mRNA transcript copy numbers per 10 ng of total RNA were quantified with real-time RT-PCR in MS patients (n=23) and normal subjects (n=18). Cultured PBMCs were sorted into T cells, B cells, monocytes, and negatively stained cells. Using sequence-specific primers the levels of (a) the common SHP-1 transcripts, (b) GAPDH mRNA levels, (c) SHP-1 promoter I transcripts, and (d) SHP-1 promoter II transcripts were quantified using real-time RT-PCR in the different cell types of both MS patients and normal subjects (*significance of P<0.05).
