Table 1 Gene profile of PBMCs of MS patients and normal subjects

From: SHP-1 deficiency and increased inflammatory gene expression in PBMCs of multiple sclerosis patients

Gene

No treatment

IL-4 treatment

 

Normal subjects

MS patients

SHP-1 siRNA

Normal subjects

MS patients

SHP-1 SiRNA

SHP-1

1.0±0.1

0.4±0.10*

0.1±0.1*

1.1±0.1

0.3±0.2†

0.1±0.1†

IL-4

1.0±0.1

3.2±0.8*

2.8±0.6*

1.8±0.2

4.1±0.5†

3.9±1.0†

IL-4-rα

1.0±0.1

0.8±0.2

1.3±0.3

1.2±0.1

1.01±0.2

1.1±0.4

CCL17

1.0±0.2

5.9±1.4*

2.8±0.5*

2.8±0.1

21.5±6.3†

6.9±1.3†

CCL11

1.0±0.3

2.2±0.4*

7.2±2.7*

2.9±0.7

6.4±2.1†

16.8±7.2†

CCR4

1.0±0.2

2.4±0.6*

1.3±0.4

1.6±0.2

2.8±0.5†

1.4±0.3

CCR8

1.0±0.2

1.5±0.3

1.3±0.5

1.2±0.1

1.8±0.2

1.5±0.2

ADAM8

1.0±0.3

3.4±0.4*

2.4±1.2*

2.6±0.6

4.8±0.6†

6.4±3.0†

LTα

1.0±0.2

3.7±1.2*

2.8±1.0*

1.8±0.2

6.2±1.6†

3.1±1.0

Arginase I

1.0±0.2

2.4±0.6*

5.1±2.1*

2.8±0.3

5.2±1.6†

10.1±5.7†

IFN-γ

1.0±0.2

1.2±0.4

1.3±0.4

0.8±0.2

0.9±0.2

1.1±0.2

IP-10

1.0±0.3

3.0±0.6*

1.9±0.4

0.7±0.4

2.9±0.5†

1.8±0.5

CXCR3

1.0±0.2

0.7±0.2

0.8+0.4

1.1+0.3

0.9+0.4

0.7+0.5

MCP-1

1.0±0.3

0.8±0.2

0.7±0.3

0.7±0.3

1.0±0.5

0.6±0.2

GAPDH

1.0±0.2

0.9±0.2

0.8±0.2

0.8±0.2

0.9±0.1

0.9±0.1

  1. *Significance of P<0.05 compared to untreated normal subjects.
  2. †Significance of P<0.05 compared to IL-4-treated normal subjects.
  3. The average is shown followed by the standard error after the ± sign.
  4. A number of genes, including several STAT6 inducible, were quantified in PBMCs of normal subjects (n=18), PBMCs of MS patients (n=23), or PBMCs of normal subjects treated with siRNA against SHP-1 (n=6) before and after 24 h treatment with IL-4. Real-time RT-PCR was used to measure the mRNA levels of SHP-1 common transcript, IL-4, IL-4 receptor α, Arginase I, the protease ADAM8, the chemokines CCL17/TARC and CCL11/eotaxin, the chemokine receptors CCR4 and CCR8, lymphotoxin-α, interferon gamma, the chemokine IP-10, the chemokine receptor CXCR3, the chemokine CCL2/MCP-1, and the housekeeping GAPDH. The actual transcript copy number of each gene is reported in relation to the untreated normal subjects, which is designated as 1.
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