Figure 1
From: Oxygen and the liberation of placental factors responsible for vascular compromise
The effect of oxygen-conditioned explant medium on MTT reduction. (a–c) MTT reduction in UtMVEC exposed to 20 (a), 6 (b) or 1% oxygen-conditioned placental explant medium (c) compared with cells exposed to oxygen-conditioned control medium for 6 h of culture. Treatment with explant-medium from each oxygen tension resulted in a significant decreases in MTT reduction compared with cells incubated with control medium (P<0.05 in all cases, Wilcoxon signed-ranks). (d) Reduction of MTT in cells exposed to 20, 6 or 1% oxygen-conditioned medium for 6 h. A significant decrease in MTT reduction occurred as the oxygen tension of the explants decreased (P<0.05, Friedman). (e) No significant differences in MTT reduction were observed following exposure of cells to explant-conditioned medium for 24 h. The ends of the boxes define the 25 and 75th percentiles, the line inside the box defines the median and the whiskers show the maximum and minimum values, N=8; *P<0.05.
