Figure 2
From: Oxygen and the liberation of placental factors responsible for vascular compromise
The effect of oxygen-conditioned explant medium on cellular ATP levels. Cellular ATP levels following exposure of UtMVEC to oxygen-conditioned placental explant medium. (a) Exposure of cells to oxygen-conditioned explant medium for 6 h resulted in significant decreases in cellular ATP levels compared with cells in control medium for each oxygen tension (P<0.01 in all cases, Wilcoxon signed-rank test; N=8). Explant medium data are represented as a percentage of cellular ATP levels in control medium (dotted line). (b) The oxygen concentration of the placental explants had a significant effect on cellular ATP levels following 6 h exposure, with a stepwise reduction in ATP as the oxygen concentration of the explant medium decreased (P<0.001 Kruskal–Wallis test followed by Dunn's post hoc analysis; N=8). (c) No significant differences in cellular ATP levels were determined following exposure to explant conditioned medium for 24 h (N=8). (d) Following immunodepletion of abundant proteins from explant conditioned medium, the reduction in ATP following exposure to hypoxic (1% O2) explant medium was still apparent (P<0.05, Mann–Whitney test; N=5). *P<0.05; **P<0.01; ***P<0.001.
