Figure 3
From: Oxygen and the liberation of placental factors responsible for vascular compromise
The effect of oxygen-conditioned explant medium on JC-1 fluorescence. (a) A reduction in the JC-1 red:green fluorescence ratio was demonstrated following exposure of cells to 5–20 μM antimycin A. Asterisks indicate values significantly different from untreated cells (P<0.05 and P<0.01, Kruskal–Wallis test and Dunn's post hoc analysis). (b) Application of explant-conditioned medium to the cells resulted in a reduction in red:green fluorescence ratios as the oxygen concentration of the explant medium decreased (P<0.001, Kruskal–Wallis test and Dunn's post hoc analysis; N=12). (c) The reduction in the red:green fluorescence ratio following cell exposure to hypoxic (1% O2) in comparison with normoxic (6% O2) explant medium was maintained following the removal of particulate material (P<0.05, Mann–Whitney test; N=6) and subsequent immunodepletion of abundant proteins (P<0.05, Mann–Whitney test; N=4). *P<0.05; **P<0.01; ***P<0.001.
