Figure 4
From: Oxygen and the liberation of placental factors responsible for vascular compromise
The effect of oxygen-conditioned explant medium on cell death. (a) TUNEL staining of cultured UtMVEC exposed to 20, 6 or 1% oxygen-conditioned explant medium for 6 h. Nuclei fluoresce red, while TUNEL-positive nuclei fluoresce green. (b) TUNEL-positive nuclei were expressed as a proportion of the total number of nuclei per field. Cells exposed to hypoxic (1% O2) explant medium displayed in increased TUNEL-positive nuclei (P<0.001 Kruskal–Wallis test and Dunn's post hoc analysis) (N=3, n=18). (c) No significant changes in LDH release occurred from cells exposed to explant medium from differing oxygen tensions (N=9). **P<0.01; ***P<0.001.
