Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Systemic and mucosal immunity induced by BCG vector expressing outer-surface protein A of Borrelia burgdorferi

Nature volume 372, pages 552–555 (1994)Cite this article

Abstract

THE bacillus Calmette–Guerin (BCG) is a live attenuated strain of Mycobacterium bovis which offers potential advantages as a vector for mucosal delivery of antigens1–3. Recombinant BCG elicits protective humoral immune responses to a variety of antigens4. Furthermore, BCG binds specifically to microfold cells5 present in the epithelium overlying lymphoid follicles throughout the mucosal immune system6–8. Here we show that a single intra-nasal vaccination with recombinant BCG expressing the outer-surface protein A antigen from B. burgdorferi9 results in a prolonged (more than one year) protective systemic IgG response and a highly sustained secretory IgA response which is disseminated throughout the mucosal immune system. Furthermore, intranasal immunization induces marked, organized lymphocyte accumulation in the proximal nasopharyngeal lymphoid tissue as well as at distal mucosal sites; the appearance and persistence of lymphoid aggregates correlates with the secretory immune responses. Thus intranasal immunization with recombinant BCG is a powerful method for inducing long-lasting secretory and systemic immune responses.

Access through your institution
Buy or subscribe

This is a preview of subscription content, access via your institution

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Jacobs, W. R. Jr, Tuckman, M. & Bloom, B. R. Nature 327, 532–535 (1987).

    Article  ADS  CAS  Google Scholar 

  2. Jacobs, W. R. Jr, Snapper, S. B., Lugosi, L. & Bloom, B. R. Curr. Top. Microbiol. Immun. 155, 153–160 (1990).

    Google Scholar 

  3. Stover, C. K. et al. Nature 351, 456–460 (1991).

    Article  ADS  CAS  Google Scholar 

  4. Stover, C. K., Bansel, G. P., Langermann, S. & Hanson, M. S. in Recombinant Vectors in Vaccine Development (ed. Brown, F.) 153–160 (Karger, Basel, 1994).

    Google Scholar 

  5. Fujimura, Y. Gastroenterologica Jap. 21, 325–335 (1986).

    Article  CAS  Google Scholar 

  6. Trier, J. S. Gastroenterol. Clin. North Am. 20, 531–547 (1991).

    CAS  PubMed  Google Scholar 

  7. Neutra, M. R. & Kraenbuhl, J. P. Physiol. Rev. 2, 134–138 (1992).

    CAS  Google Scholar 

  8. Langermann, S. & Amerongen, H. M. Curr. Opin. Gastroenterol. 8, 983–987 (1993).

    Google Scholar 

  9. Bergstrom, S., Bundoc, V. & Barbour, A. G. Molec. Microbiol. 3, 479–486 (1989).

    Article  CAS  Google Scholar 

  10. Stover, C. K. et al. J. exp. Med. 178, 197–209 (1993).

    Article  CAS  Google Scholar 

  11. Simon, M. M. et al. J. infect. Dis. 164, 123–132 (1991).

    Article  CAS  Google Scholar 

  12. Fikrig, E., Barthold, S. W., Kantor, F. S. & Flavell, R. Science 250, 553–556 (1990).

    Article  ADS  CAS  Google Scholar 

  13. Sadziene, A., Thompson, P. A. & Barbour, A. G. J. infect. Dis. 167, 165–172 (1993).

    Article  CAS  Google Scholar 

  14. Schwann, T. G. et al. J. clin. Microbiol. 26, 893–895 (1988).

    Google Scholar 

  15. Abraham, E. & Shah, S. J. Immun. 149, 3719–3726 (1992).

    CAS  PubMed  Google Scholar 

  16. Vajdy, M. & Lycke, N. Y. Immunology 75, 488–492 (1992).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. O'Hagan, D. T. et al. Vaccine 11, 149–154 (1993).

    Article  CAS  Google Scholar 

  18. Quiding, M. et al. J. clin. Invest. 88, 143–148 (1991).

    Article  CAS  Google Scholar 

  19. Haneberg, B. et al. Infect. Immun. 62, 15–23 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

  20. McDermott, M. R. & Bienenstock, J. J. Immun. 122, 1892–1898 (1979).

    CAS  PubMed  Google Scholar 

  21. Fuhrman, J. A. & Cebra, J. J. J. exp. Med. 153, 534–544 (1981).

    Article  CAS  Google Scholar 

  22. Czerkinsky, C. et al. Proc. natn. Acad. Sci. U.S.A. 84, 2449–2453 (1987).

    Article  ADS  CAS  Google Scholar 

  23. Czerkinsky, C. et al. J. immun. Meth. 65, 109–121 (1983).

    Article  CAS  Google Scholar 

  24. Sedgwick, J. D. & Holt, P. G. J. immun. Meth. 57, 301–307 (1983).

    Article  CAS  Google Scholar 

  25. Young, J. T. Fund. appl. Toxicol. 1, 309–312 (1981).

    Article  CAS  Google Scholar 

  26. Kuper, C. F. et al. Immun. Today 13, 219–225 (1992).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. C. Kendall Stover

    Present address: PathoGenesis Corporation, Seattle, Washington, 98119, USA

Authors and Affiliations

  1. Medlmmune Inc., Gaithersburg, Maryland, 20878, USA

    Solomon Langermann, Susan Palaszynski, C. Kendall Stover & Scott Koenig

  2. Department of Microbiology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78284, USA

    Ariadna Sadziene

Authors
  1. Solomon Langermann
    View author publications

    Search author on:PubMed Google Scholar

  2. Susan Palaszynski
    View author publications

    Search author on:PubMed Google Scholar

  3. Ariadna Sadziene
    View author publications

    Search author on:PubMed Google Scholar

  4. C. Kendall Stover
    View author publications

    Search author on:PubMed Google Scholar

  5. Scott Koenig
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Langermann, S., Palaszynski, S., Sadziene, A. et al. Systemic and mucosal immunity induced by BCG vector expressing outer-surface protein A of Borrelia burgdorferi. Nature 372, 552–555 (1994). https://doi.org/10.1038/372552a0

Download citation

  • Received:

  • Accepted:

  • Issue date:

  • DOI: https://doi.org/10.1038/372552a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing