Abstract
Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin VK segment can be replaced by human β-globin or prokaryotic neo or gpt genes without affecting the rate of hypermutation; the V gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes in vivo could find more general applications in biology.
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Yélamos, J., Klix, N., Goyenechea, B. et al. Targeting of non-lg sequences in place of the V segment by somatic hyper mutation. Nature 376, 225–229 (1995). https://doi.org/10.1038/376225a0
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DOI: https://doi.org/10.1038/376225a0
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