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Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex

Nature volume 376, pages 313–320 (1995)Cite this article

Abstract

The crystal structure of the human cyclinA-cyclin-dependent kinase2 (CDK2)-ATP complex has been determined at 2.3 A resolution. CyclinA binds to one side of CDK2's catalytic cleft, inducing large conformational changes in its PSTAIRE helix and T-loop. These changes activate the kinase by realigning active site residues and relieving the steric blockade at the entrance of the catalytic cleft.

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Authors and Affiliations

  1. Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York, 10021, USA

    Philip D. Jeffrey, Alicia A. Russo & Nikola P. Pavletich

  2. Cell Biology and Genetics Program and the Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, New York, 10021, USA

    Kornelia Polyak & Joan Massagué

  3. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, 10021, USA

    Emma Gibbs & Jerard Hurwitz

Authors
  1. Philip D. Jeffrey
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  2. Alicia A. Russo
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  3. Kornelia Polyak
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  4. Emma Gibbs
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  6. Joan Massagué
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  7. Nikola P. Pavletich
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Jeffrey, P., Russo, A., Polyak, K. et al. Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex. Nature 376, 313–320 (1995). https://doi.org/10.1038/376313a0

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