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Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1

Nature volume 376, pages 745–753 (1995)Cite this article

Abstract

The crystal structure of mammalian protein phosphatase-1, complexed with the toxin microcys-tin and determined at 2.1 Å resolution, reveals that it is a metalloenzyme unrelated in architecture to the tyrosine phosphatases. Two metal ions are positioned by a central β-α-β-α-β scaffold at the active site, from which emanate three surface grooves that are potential binding sites for substrates and inhibitors. The carboxy terminus is positioned at the end of one of the grooves such that regulatory sequences following the domain might modulate function. The fold of the catalytic domain is expected to be closely preserved in protein phosphatases 2A and 2B (calcineurin).

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Authors and Affiliations

  1. Howard Hughes Medical Institute, 1230 York Avenue, New York, New York, 10021, USA

    Jonathan Goldberg & John Kuriyan

  2. The Rockefeller University, 1230 York Avenue, New York, New York, 10021, USA

    Hsien-bin Huang, Young-guen Kwon, Paul Greengard & Angus C. Nairn

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  1. Jonathan Goldberg
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  2. Hsien-bin Huang
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  5. Angus C. Nairn
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  6. John Kuriyan
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Goldberg, J., Huang, Hb., Kwon, Yg. et al. Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1. Nature 376, 745–753 (1995). https://doi.org/10.1038/376745a0

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