Using recent findings by Tabor and Richardson, a novel thermostable DNA polymerase that generates sequence data comparable with T7 Sequenase DNA polymerase has been developed for improved base calling and longer reads.
Enjoying our latest content?
Log in or create an account to continue
- Access the most recent journalism from Nature's award-winning team
- Explore the latest features & opinion covering groundbreaking research
or
References
Tabor, S. & Richardson, C. C. Proc. natn. Acad. Sci. U.S.A. 92, 6339–6343 (1995).
Sanger, F., Niklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Samols, S. B. & Fuller, C. W. Comments 21, 57–78, 1995). United States Biochemical, Cleveland, Ohio. USA.
Klenow, H., Overgaard-Hansen, K. & Patkar-S, A. Eur. J. Biochem. 22, 371–381 (1971).
Joyce, C. M., Kelley, W. S. & Grindley, N. D. J. Biol. Chem. 257, 1958–1964 (1982).
Barnes, W. M. Gene 112, 29–35 (1992).
Lawyer, F. C. et al. PCR Meth. Appl. 2, 275–287 (1993).
Carothers, A. M., Urlaub, G., Mucha, J., Grunberger, D. & Chasin, L. A. Biotechniques 7, 494–496, 498–499 (1989).
Levedakou, E. N., Landegren, U. & Hood, L. E. Biotechniques 7, 438–442 (1989).
Murray, V. Nucleic Acids Res. 17, 8889 (1989).
Lee, J. S. DNA Cell Biol. 10, 67–73 (1991).
Axelrod, J. D. & Majors, J. Nucleic Acids Res. 17, 171–183 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reeve, M., Fuller, C. A novel thermostable polymerase for DNA sequencing. Nature 376, 796–797 (1995). https://doi.org/10.1038/376796a0
Issue date:
DOI: https://doi.org/10.1038/376796a0
This article is cited by
-
Advanced preparation of fragment libraries enabled by oligonucleotide-modified 2′,3′-dideoxynucleotides
Communications Chemistry (2022)
-
Archaeal DNA polymerases in biotechnology
Applied Microbiology and Biotechnology (2015)
-
Inverse correlation of HER2 with MHC class I expression on oesophageal squamous cell carcinoma
British Journal of Cancer (2010)
-
Initial sequencing and analysis of the human genome
Nature (2001)
-
Efficient identification of point mutations by automated DNA sequencing of artificial heterozygote samples
Molecular Biotechnology (1998)
