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Protection against mycoplasma infection using expression-library immunization

Nature volume 377, pages 632–635 (1995)Cite this article

Abstract

AS is evident from the human immunodeficiency virus epidemic, there is no systematic method for producing a vaccine. Genetic immunization1is a new approach to vaccine production that has many of the advantages of live/attenuated pathogens but no risk of infection. It involves introducing DNA encoding a pathogen protein into host cells and has shown promise in several disease models2á¤-13. Here we describe a new method for vaccine development, expression-library immunization, which makes use of the technique of genetic immunization and the fact that all the antigens of a pathogen are encoded in its DNA. An expression library of pathogen DNA is used to immunize a host thereby producing the effects of antigen presentation of a live vaccine without the risk. We show that even partial expression libraries made from the DNA of Mycoplasma pulmonis, a natural pathogen in rodents, provide protection against challenge from the pathogen. Expression library immunization may prove to be a general method for vaccination against any pathogen.

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Authors and Affiliations

  1. Department of Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75235-8573, USA

    Michael A. Barry & Stephen Albert Johnston

  2. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, 75235-8573, USA

    Stephen Albert Johnston

  3. Division of Comparative Medicine, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, 75235-9073, USA

    Wayne C. Lai

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  1. Michael A. Barry
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  2. Wayne C. Lai
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  3. Stephen Albert Johnston
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Barry, M., Lai, W. & Albert Johnston, S. Protection against mycoplasma infection using expression-library immunization. Nature 377, 632–635 (1995). https://doi.org/10.1038/377632a0

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