Figure 3 | Laboratory Investigation

Figure 3

From: Inhibition of Experimental Allergic Encephalomyelitis with an Antibody that Recognizes a Novel Antigen Expressed on Lymphocytes, Endothelial Cells, and Microglia

Figure 3

Immunoprecipitation of 4A2 from an endothelial cell line and freshly derived lymph node cells. A, Immunoprecipitate from rat CNS endothelium (GP8. E4) in cell culture made with TLD-4A2, separated by two-dimensional SDS gel electrophoresis (non-reduced/reduced). The 4A2 protein appears as a major spot at 50 kD with a minor spot at 46 kD. The molecular weights are the same in both first (non-reduced) and second (reduced) dimensions. The major contaminant of the precipitate is IgG (160 kD non-reduced, 56 kD and 26 kD reduced). B, 4A2 immunoprecipitate (non-reduced) from brain endothelium. Major bands are at 50 kD and 46 kD, with high molecular weight contaminants. C, 4A2 immunoprecipitate (reduced) from rat lymph node cells with a major band at 50 kD.

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