Figure 1
From: Immunostaining and Laser-Assisted Cell Picking for mRNA Analysis
A to D, Laser-assisted cell picking in frozen tissue after immunohistochemical staining. Kidney, stained with the RECA-1 antibody (rat pan-endothelial), alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP). A, A glomerulum is selected. B, Removal of the adjacent tissue by the UV-laser and reduction of the stained glomerulus to obtain a cell cluster with approximately 20 nuclei. C, The cell cluster is harvested with a needle. D, The cell cluster adheres tightly to the needle and is lifted. E to G, Rapid immunostaining; examples of APAAP immunohistochemical and FITC indirect immunofluorescence staining with rapid protocol. Cryosections of lung and kidney are shown before laser microdissection of alveolar or glomerular cells. Original magnifications × 40. E, Rapid immunofluorescence staining of the lung with the ED1 (rat macrophage/monocyte) antibody. Arrows show the specifically stained alveolar macrophages. F, Rapid immunohistochemical staining of the lung with the ED1 (rat macrophage/monocyte) antibody. G, Rapid immunofluorescence staining of the kidney with the RECA-1 (rat pan-endothelial) antibody. Clear identification of RECA-1 positive cells.
