Figure 2
From: Immunostaining and Laser-Assisted Cell Picking for mRNA Analysis
Typical example of RT-PCR analysis for PBGD mRNA after laser-assisted cell picking. Immunofluorescence staining was done with tissue sections from a formalin-fixed lung. Oligocellular clusters with approximately 20 nuclei per sample were isolated. Eight samples (lanes 2 to 9) were digested with proteinase K, 5 samples (lanes 11 to 15) were not. Intron spanning primers were used for amplification. PBGD RT-PCR product with 126 bp length was generated. Band intensity varied slightly, but was not related to any of the factors investigated. Lane 16, sample without RT; lanes 1, 10, and 17, ΦX174 HinfI. 2.5% agarose gel/TBE gel stained with ethidium bromide.
