Figure 3 | Laboratory Investigation

Figure 3

From: Loss of Cell-Cell Contact Is Induced by Integrin-Mediated Cell-Substratum Adhesion in Highly-Motile and Highly-Metastatic Hepatocellular Carcinoma Cells

Figure 3

Association of E-cadherin with catenins in KYN-2 cells. KYN-2 cells were cultured on dishes coated with polyHEMA (sus.) or collagen Type I (adh.). A, E-cadherin and associated proteins collected with anti-E-cadherin antibody and detected using antibodies against E-cadherin, α-catenin, β-catenin, plakoglobin. B, α-catenin and associated proteins collected with anti-α–catenin polyclonal antibody and detected using anti-E-cadherin or α-catenin antibodies. C, Expression of E-cadherin, α-catenin, β-catenin, plakoglobin, and p120CAS in whole cell lysates analyzed by immunoblotting. D, E-cadherin and associated proteins collected with anti-E-cadherin antibody and detected using anti-E–cadherin or anti-α–catenin antibodies. KYN-2 cells were cultured on dishes coated with collagen Type I in the presence of inhibitory monoclonal antibodies against β1 and β5 integrins (Lane 2) or nonimmunized mouse IgG (Lane 3) or without antibodies (Lane 1). Each result is representative of a total of three experiments.

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