Figure 4 | Laboratory Investigation

Figure 4

From: Loss of Cell-Cell Contact Is Induced by Integrin-Mediated Cell-Substratum Adhesion in Highly-Motile and Highly-Metastatic Hepatocellular Carcinoma Cells

Figure 4

Association and activation of c-Src in KYN-2 cells. KYN-2 cells were cultured on dishes coated with polyHEMA (sus.) or collagen Type I (adh.). A, Immunoblotting with anti-phosphotyrosine monoclonal antibody of KYN-2 cells. Molecular mass positions (kDa) are shown on the left. B, Immunoprecipitates collected with anti-c-Src polyclonal antibody analyzed with anti-c-Src, E-cadherin, β-catenin, or phosphotyrosine monoclonal antibodies, and immunoprecipitates collected with anti-phosphotyrosine polyclonal antibody analyzed with anti-c-Src monoclonal antibody. C, Tyrosine kinase activity of immunoprecipitates collected with anti-c-Src polyclonal antibody analyzed as described in “Materials and Methods.” Data represent mean ± sd of the results of three studies carried out in triplicate. D, Immunoprecipitates collected with anti-c-Src polyclonal antibody analyzed with anti-phosphotyrosine monoclonal antibody. KYN-2 cells were cultured on dishes coated with collagen Type I in the presence of inhibitory monoclonal antibodies against β1 and β5 integrins (Lane 2) or nonimmunized mouse IgG (Lane 3) or without antibodies (Lane 1). Each result of A, B, and D is representative of a total of three experiments.

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