Table 5 Blocking of Cytotoxicity of CD4+T Cells Toward IFN-γ-Stimulated Thyrocytes

From: CD4+ T Cell-Mediated Cytotoxicity Toward Thyrocytes: The Importance of Fas/Fas Ligand Interaction Inducing Apoptosis of Thyrocytes and the Inhibitory Effect of Thyroid-Stimulating Hormone

Treatment of thyrocytes with mAb/hFas-Fc

Concentration of mAb/hFas-Fc (μg/ml)

 

0

2.5

5

10

20

(−)

56.5 ± 4.6

    

control IgG

 

NT

56.9 ± 4.9

57.2 ± 5.1

58.0 ± 5.0

anti-HLA-ABC

 

NT

54.9 ± 4.6

55.0 ± 5.1

57.0 ± 3.9

anti-HLA-DR and -DQ

 

NT

18.8 ± 1.1

12.7 ± 1.0

12.2 ± 0.9

hFas-Fc

 

NT

35.9 ± 2.1

27.1 ± 1.5

27.9 ± 1.9

anti-FasL

 

26.9 ± 1.7

25.5 ± 1.9

24.9 ± 1.5

NT

anti-CD54

 

NT

33.7 ± 1.7

28.5 ± 1.7

28.0 ± 1.4

anti-CD58

 

NT

39.9 ± 2.0

33.7 ± 2.2

33.0 ± 1.5

anti-CD54 and -CD58

 

NT

18.1 ± 1.0

11.9 ± 1.2

10.9 ± 1.3

anti-TNF-α

 

NT

55.9 ± 4.2

52.9 ± 3.9

54.0 ± 2.9

anti-IL-1β

 

NT

54.3 ± 3.2

54.0 ± 4.2

54.7 ± 2.7

  1. Thyrocytes from patients with Graves’ disease were cultured with rIFN-γ (500 IU/ml) for 72 hours. After incubation, the cells were labeled with 51Cr and further incubated with CD4+T cells (E/T ratio of 10) in the presence of 1 μg/ml of SEB with or without various mAb/hFas-Fc for 18 hours. After incubation, cytotoxicity of CD4+T cells toward IFN-γ-stimulated thyrocytes was examined as described in the text. Results are the mean ± sd of four experiments. NT, not tested.
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