Table 6 FasL Expression of CD4+ T Cells Cocultured with Thyrocytes

From: CD4+ T Cell-Mediated Cytotoxicity Toward Thyrocytes: The Importance of Fas/Fas Ligand Interaction Inducing Apoptosis of Thyrocytes and the Inhibitory Effect of Thyroid-Stimulating Hormone

Treatment of thyrocytes

FasL expression of CD4+ T cells (%)

IFN-γ

SEB

mAb/TSH

 

(−)

(−)

(−)

2.8 ± 0.3

(−)

(−)

control IgG

2.9 ± 0.2

(−)

(+)

(−)

4.4 ± 0.8

(−)

(+)

control IgG

4.4 ± 0.9

(+)

(−)

(−)

3.1 ± 0.3

(+)

(−)

control IgG

3.0 ± 0.3

(+)

(+)

(−)

28.2 ± 3.0*

(+)

(+)

control IgG

28.0 ± 2.7*

(+)

(+)

anti-HLA-DR and -DQ

9.0 ± 0.8**

(+)

(+)

anti-CD54 and -CD58

6.8 ± 0.5**

(+)

(+)

TSH

27.4 ± 2.5**

  1. Thyrocytes were incubated with or without rIFN-γ (500 IU/ml) for 72 hours. After incubation, the cells were incubated with purified CD4+ T cells at the E/T ratio of 10 for 18 hours in the presence or absence of 1 μg/ml of SEB with or without various mAb (10 μg/ml) or TSH (10 mIU/ml). FasL expression of CD4+ T cells was examined as described in the text.
  2. *p < 0.01 vs. IFN-γ-stimulated thyrocytes in the absence of SEB.
  3. **p < 0.01 vs. IFN-γ-stimulated thyrocytes in the presence of SEB with control IgG or in the absence of mAb. Results shown are the mean ± sd from four experiments.
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