Figure 5 | Laboratory Investigation

Figure 5

From: Metallothionein Inhibits Myocardial Apoptosis in Copper-Deficient Mice: Role of Atrial Natriuretic Peptide

Figure 5Figure 5

Effect of MT on atrial natriuretic peptide- (ANP)-induced apoptosis in cultured primary mouse cardiac myocytes. Cultures of non-transgenic (A and B) and transgenic (C and D) cardiomyocytes were exposed to 0.1 μm ANP for 4 hours (B and D) and the apoptotic cells were detected by a TUNEL assay (A to D). The effect of MT on ANP concentration-dependent apoptotic effect was detected by the same TUNEL assay and semi-quantified by counting the apoptotic nuclei against normal nuclei (E). Data were obtained from 3 cultures for each treatment and are expressed as mean ± sd. * indicates significantly different from non-TG cultures. Original magnification ×370.

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