Table 1 Overview of Endothelial Cell Isolation from Iliac Vessels

Blood Vessel^a	EC surface (mm²)	EC quality (1–10)	Days in Culture	Total Number of Cells (10⁶)	% vWF Pos. Cells^b	% CD31 Pos. Cells^b
Chymotrypsin for 60 min.
V1	410	6	18	0.30	±	+
V5	840	7	19	0.27	+	+++
V2	600	6	20	0.25	−	±
V9	840	8	29	0.15	−	±
V4	10	2	36	none
V3	n.d.	7	49^c	0.35	++	+++++
A4	70	4	13	0.45	++++	+++++
A1	120	5	18	0.30	±	±
A2	90	3	20	0.14	−	+
Collagenase for 60 min.
V8	520	8	5	0.88	+++++	+++++
V9	1260	8	13	0.90	+++++	+++++
V10	1050	7	26	none
Collagenase for 30 min.
V12	650	7	10	0.55	++++	+++++
V16	550	4	12	0.80	++++	+++++
V14	1260	6	14	0.50	+++	+++++
V13	140	2	35^c	0.45	++++	+++++
A15	420	3	5	0.40	+++	+++++
A14	400	5	14	0.30	+	+++++
A11	500	7	17	0.45	+	+++++
A12	440	6	18	0.55	+	+++++
A16	420	7	21	0.45	−	−
A13	650	8	35	none

Data are sorted for days in culture.
^aVeins (V) and arteries (A) are numbered in order of isolation.
^bThe percentage of vWF and CD3 positive cells was scored as follows: − (negative), ± (<1%), + (1–20%), ++ (20–40%), +++ (40–60%), ++++ (60–80%), +++++ (80–100%).
^cOutgrowth of one endothelial cell cluster, probably derived from one single isolated endothelial cell.
n.d. = not determined.

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