Figure 3

Endothelial and inducible nitric oxide synthase (eNOS and iNOS) immunohistochemistry. (a) A representative glomerulus from a normal rat shows occasional immunoreactivity for iNOS in glomerular cells (arrows, original magnification × 400). (b and c) iNOS immunoreactivity 180 minutes after endotoxin administration reveals intense immunoreactivity in glomerular cells, arterioles, peritubular capillaries, and scattered interstitial cells (b: original magnification, × 200; c: original magnification, × 600). (d) Representative photomicrograph from control rats reveals weak eNOS immunoreactivity in occasional glomerular cells and strong immunoreactivity in arterioles and peritubular capillaries (original magnification, × 200). (e) Representative photomicrograph from rats 180 minutes after endotoxin injection demonstrates diffuse peritubular capillary and occasional glomerular cell immunoreactivity (inset) for eNOS. Although the intensity of eNOS immunoreactivity is slightly less in the TMA group, the current methodology is not sensitive enough to detect differences in the course of TMA (original magnification, × 200).