Figure 5
From: Alterations of the Rat Mesentery Vasculature in Experimental Diabetes
Histograms demonstrating the distribution of gold particles across the endothelial and mesothelial basement membranes of diabetic and age-matched control rats. R = d/D represents the relative distance separating the gold particles from the abluminal plasma membrane (PM). A value of 0 localizes the gold particles directly at the PM, whereas a value of 1.0 is attributed to the outermost particles. For the endothelial basement membrane, the evaluation was performed on 74 profiles (300 gold particles) and 71 profiles (184 gold particles) for diabetic and control groups, respectively. For the mesothelial basement membrane, the evaluation was performed on 62 mesothelial profiles (318 particles) and 68 mesothelial profiles (252 gold particles) from diabetic and control groups, respectively. The distribution of BSA across the endothelial basement membrane is almost uniform for both the diabetic and control groups, whereas for the mesothelium in diabetic condition, the labeling for albumin is confined to the interstitial part of the mesothelial basement membrane. The control group does not display such an asymmetric distribution.
