Figure 1 | Laboratory Investigation

Figure 1

From: Analysis of the IGF Axis in Preneoplastic Hepatic Foci and Hepatocellular Neoplasms Developing after Low-Number Pancreatic Islet Transplantation into the Livers of Streptozotocin Diabetic Rats

Figure 1

Insulin-like growth factor-I (IGF-I) (A), IGF binding protein-1 (IGFBP-1), and IGFBP-4 (B) mRNA expression of total RNA from the left (free of transplants) part of the livers (control) and from glycogen-storing foci (GSF) of the same liver 7 days after islet transplantation. Total RNA was size selected by 2% agarose gel electrophoresis. Membranes were hybridized using a labeled antisense RNA or a cDNA specific for rat IGF-I, IGFBP-1, or IGFBP-4, as indicated. The sizes of the hybridization bands are indicated on the right. To ensure equal loading of RNA, membranes were stripped and rehybridized with an oligonucleotide complementary to 28 S rRNA (lowest panel).

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