Figure 5 | Laboratory Investigation

Figure 5

From: Analysis of the IGF Axis in Preneoplastic Hepatic Foci and Hepatocellular Neoplasms Developing after Low-Number Pancreatic Islet Transplantation into the Livers of Streptozotocin Diabetic Rats

Figure 5

IGF-I and IGF-II (A), IGF-IR and IGF-II/M6PR (B), and IGFBP-1 and IGFBP-4 (C) mRNA expression in hepatocellular carcinomas (HCC) that developed in the right part of the livers after low-number pancreatic islet transplantation. The left parts of the same livers, which were free of transplants and of tumors, were used as controls. Total RNA isolated from controls and HCC was separated by a 2% agarose gel electrophoresis. Membranes were hybridized using labeled probes specific for IGF-I and IGF-II (A), for IGF-IR and IGF-II/M6PR (B), and for IGFBP-1 and IGFBP-4 (C), as indicated. The sizes of the hybridization bands are indicated on the right. To ensure equal loading of RNA, membranes were stripped and rehybridized with an oligonucleotide complementary to 28 S rRNA (lowest panel).

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