Figure 6

ISH with [35S]-labeled antisense RNA probes in parallel sections of a HCC (left part of the figures), 24 months after islet transplantation. A strong increase of IGF-II (B) and decreases of IGF-I (A) and IGFBP-1 (D) mRNA expression compared with the neighboring nontumorous liver tissue (right part of the images) is seen. IGFBP-1 (C) mRNA was present at approximately the same levels in tumorous and nontumorous liver tissue. Dark field illumination. Original magnification, ×40.