Table 1 Primer Sets for RT-PCR
From: Expression of Interleukin-7 and Its Receptor in Thyroid Lymphoma
Sequence forward/reverse | cDNA (bp) | Ta (°C) | Cycles | ||
|---|---|---|---|---|---|
IL-7 | Up | 5′TTTTATTCCGTGCTGCTCGC3′ | 429 | 66 | 37 |
Down | 5′GCCCTAATCCGTTTTGACCA3′ | ||||
IL-7R | Up | 5′GAAGGTTGGAGAAAAGAGTC3′ | 418 | 52 | 30 |
Down | 5′CAAAATGCTGATGGTTAGTAAG3′ | ||||
γ-chain | Up | 5′ACGGGAACCCAGGAGACAGG3′ | 275 | 52 | 30 |
Down | 5′AGCGGCTCCGAACACGAAAC3′ | ||||
β-actin | Up | 5′CTTCCTTCCTGGGCATGGAG3′ | 315 | 66 | 25 |
Down | 5′TGGAGGGGCCGGACTCGTCA3′ | ||||
β-actin competitor | Up | 5′CTTCCTTCCTGGGCATGGAG-TACGGTCATCATCTGACAC3′ | 339 | 66 | 25 |
Down | 5′TGGAGGGGCCGGACTCGTCA-CGCCATCCTGGGAAGACTCC3′ |
