Figure 1
From: E-Cadherin and Cadherin-Associated Cytoplasmic Proteins Are Expressed in Murine Mast Cells
FACS analysis of E-, N-, and P-cadherin on bone marrow-derived cultured mast cells (BMMC) and peritoneal mast cells (PMC). a and b, Cells were stained with PE-conjugated anti-c-kit mAb. c and d, Cells were incubated with anti-E-cadherin mAb (ECCD)-2, followed by FITC-labeled antirat IgG1. e to j, Cells were first incubated with rat mAb against mouse CD16/32. After washing, cadherins were detected by incubation with biotinylated ECCD-2, anti-N-cadherin mAb (GC-4), or anti-P-cadherin mAb (PCD-1), followed by FITC-conjugated streptavidin. For control staining, irrelevant isotype-matched control antibodies were employed. Open histogram, isotype-matched control; solid histogram, c-kit or cadherins. The results shown are representative of five experiments.
