Figure 9
From: E-Cadherin and Cadherin-Associated Cytoplasmic Proteins Are Expressed in Murine Mast Cells
Effects of anti-E-cadherin antibodies and synthetic decapeptides on adhesion of BMMC to F9-cell monolayers. BCECF-AM-labeled BMMC were preincubated for 30 minutes with 20 μg/ml of indicated antibodies or peptides and then co-cultured with nonlabeled F9 cell monolayers in the presence of the same amounts of antibodies or peptides. Percentages of binding of BMMC were determined by measuring fluorescence intensity after removing unbound cells in reference to the standard curve produced using known numbers of labeled BMMC. A, BMMC binding rate to F9 cells in the presence of ECCD-1, ECCD-2, or control rat IgG2b. B, BMMC binding rate to F9 cells in the presence of E-cadherin peptide 159-68, E-cadherin peptide 232-41, or P-cadherin peptide 175-84. Bars represent sd of four cultures. */** Significantly different from the corresponding value for isotype-matched control IgG or E-cadherin peptide 159-68 (*p< 0.05, **p < 0.01).
