Figure 2

β-globin, immunoglobulin heavy chain gene (IgH), and major breakpoint region/joining region (MBR/JH) PCR of microdissected follicular and monocytoid B-cell components from hematoxylin-eosin stained sections. A, β-globin PCR. Lanes 1–8, Follicular (F) and monocytoid B-cell (M) components of Cases I to IV. B, IgH PCR. Upper Lane 1, DNA fragment size marker (L). Upper Lane 2, Negative DNA control from reactive human tonsil (−). Upper Lane 3, Positive DNA control from the SUDHL-6 cell line (+). Upper Lanes 4–7, Follicular (F) and monocytoid B-cell (M) components from Case I, each in duplicate. Upper Lane 8, Control without template DNA (H₂O only). Lower Lane 1, DNA fragment size marker (L). Lower Lane 2, Negative DNA control from reactive human tonsil (−). Lower Lane 3, Positive DNA control from the SUDHL-6 cell line (+). Lower Lanes 4–7, Follicular (F) and monocytoid B-cell (M) components from Case II, each in duplicate. Lower Lane 8, Control without template DNA (H₂O only). C, IgH PCR. Lane 1, DNA fragment size marker (L). Lane 2, Negative DNA control from reactive human tonsil (−). Lane 3, Positive DNA control from the SUDHL-6 cell line (+). Lane 4, Control without template DNA (H₂O only). Lanes 5–8, Follicular (F) and monocytoid B-cell (M) components from Case III, each in duplicate. D, IgH PCR. Lane 1, DNA fragment size marker (L). Lanes 2–5, Follicular (F) and monocytoid B-cell (M) components from Case IV, each in duplicate. E, MBR/JH translocation PCR. Lane 1, DNA fragment size markers (L). Lane 2, Negative DNA control from reactive human tonsil (−). Lane 3, Positive DNA control from the SUDHL-6 cell line (+). Lanes 4–11, Follicular (F) and monocytoid B-cell (M) components of Cases I to IV.