Table 2 IgH PCR Product Sequencing: Comparison of CDRIII DNA Sequences a

From: Follicular Lymphoma with Monocytoid B-Cell Proliferation: Molecular Assessment of the Clonal Relationship between the Follicular and Monocytoid B-Cell Components

CASE

3′ END OF VH

N

DH

N

JH

   

DHQ52

 

JH4

GERMLINE

ACTGT

 

CTGAGCTGAGAA

 

ACTTTTGACTACTGGGGCCAAGGAACCCTGGTC

 IF

 

GAG

T---A-G-----

GGA

-----------------G-----------

 IM

 

---

------------

---

------------------------------

   

DA1

 

JH1

GERMLINE

TGT

 

TGACTACAGTAA

 

CAGGGCACCCTGGTCACCGTC

 IIIF

 

GCAAAA

--G-CC----G-

GTCACC

-----TT-----C-C-A----

 IIIM

 

------

----T*--A*--

-----

---------------------

  1. CDRIII, complementarity determining region III; M, monocytoid B-cell component; F, follicular component; V, variable region; N, non-templated nucleotides; D, diversity region; J, joining region; T*, A*, single base substitutions in the monocytoid B-cell component of Case III.
  2. a a CDRIII DNA sequences from follicular and monocytoid B-cell components of Cases I and III. Germline sequences and diversity and joining region designations are included with each case. Sequences from follicular and monocytoid B-cell components are aligned with their respective germline DH and JH segments. Dashes (---) represent identity with the germline sequence.
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