Figure 4
From: Conversion of Human Colonic Adenoma Cells to Adenocarcinoma Cells Through Inflammation in Nude Mice
Confirmation of human origin of the adenocarcinoma cell lines by RT-PCR analysis. cDNA was synthesized from mRNA samples extracted from parental FPCK-1–1, FPCK-1–1-derived adenocarcinoma lines, human colorectal tumor cell lines, mouse plastic plate-reactive fibroblast, and mouse fibrosarcoma cell line. Each cDNA was amplified with specific primer sets for human or mouse thymosin β4. Lane 1, øX174-HaeIII digest DNA marker; Lane 2, parental FPCK-1–1 cells; Lanes 3–6, FPCK-1–1-derived cell lines (FPCKpP1-1, FPCKpP1-2, FPCKpP1-3 and FPCKpP1-4 cell line, respectively; Lane 7, DLD-1 cell line; Lane 8, HCT-116 cell line; Lane 9, plastic plate-reactive mouse fibroblast; Lane 10, mouse fibrosarcoma cell line; Lane 11, negative control containing all the reaction mixtures except cDNA; Lane 12, øX174-HaeIII digest DNA marker.
