Table 1 Tumorigenicity of FPCK-1–1 Cells Implanted in Suspension, Attached to a Plastic Plate, or into a Preinserted Gelatin Sponge in KSN Nude Mice

From: Conversion of Human Colonic Adenoma Cells to Adenocarcinoma Cells Through Inflammation in Nude Mice

 

Tumorigenicity (no. of mice with tumor/no. of mice injected)a

 

5 × 106 cells in saline suspension

1 × 105 cells attached to plastic plate

5 × 106 cells into preinserted gelatin sponge

Cells

Exp. 1

Exp. 2

Total

Exp. 1

Exp. 2

Total

Mean latency period (days)b

Plate alone

Exp. 1

Exp. 2

Total

Mean latency period (days)b

Sponge alone

FPCK-1–1

0/5

0/7

0/12

7/10

6/10

13/20

107 ± 7

—

1/4

0/8

1/12

149

—

None

—

—

—

—

—

—

—

0/8c

—

—

—

—

0/4c

  1. aAdenoma cells were implanted in PBS suspension or attached to plastic plates sc in 6- to 8-week-old KSN nude mice; and 5 × 106 cells were implanted sc into preinserted gelatin sponge. The mice were observed for 12 months.
  2. bLatency period = days before mean tumor diameter reached >3 mm.
  3. cAfter plastic plate or gelatin sponge was implanted sc without adenoma cells, none of the mice (n = 8 and n = 4, respectively) developed tumors during observation. The nontumor-bearing mice were examined for an additional 8 months, during which no other tumors were observed.
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