Table 2 Comparison of Tumorigenicity in Nude Mice and In Vitro Characteristics of the Parental FPCK-1–1 Cell Line and the Tumors Arisen from FPCK-1–1 Cells Implanted Attached to a Plastic Plate or Injected into a Preinserted Gelatin Sponge

From: Conversion of Human Colonic Adenoma Cells to Adenocarcinoma Cells Through Inflammation in Nude Mice

  

In vivo

In vitro

Cell linesa

Cell line established from FPCK-1–1 cells implanted with

Tumorigenicity (No. of mice with tumor/no. of mice injected)b

VEGF production pg/mlc

Doubling time (hours)

Plating efficiency (%)d

Number of colonies per 4 × 104 cells in soft agare

  

5 × 106

1 × 106

    

FPCK-1–1

—

0/11

0/9

3246 ± 293

38.5

20.8

0, 1, 2

FPCKpP1–1

Plastic plate

2/2*

0/2g

7833 ± 471

27.1

6.4

>250, >250, >250

FPCKpP1–2

Plastic plate

2/2*

2/2*

7082 ± 1350

31.3

13.5

1, 1, 2

FPCKpP1–3

Plastic plate

2/2*

6/6*

5303 ± 78

31.5

13.2

6, 10, 11

FPCKpP1–4

Plastic plate

NTf

2/2*

6075 ± 642

43.6

17.6

0, 3, 5

FPCKpP1–6

Plastic plate

3/3*

1/2**

8007 ± 552

NTf

18.3

6, 15, 15

FPCKsP1–1

Gelatin sponge

4/4*

NTf

7465 ± 35

23.2

3.1

3, 3, 5

  1. aFPCK-1–1 cells (1 × 105) were attached to a plastic plate in culture and implanted sc, or 5 × 106 cells were injected sc into preinserted gelatin sponge in KSN nude mice. The arising tumors were cultured separately and named FPCKpP1–1 serially to FPCKpP1–6 and FPCKsP1–1.
  2. bKSN nude mice were injected sc with FPCK-1–1 cells or FPCKpP1 cells without plastic plate.
  3. cVEGF levels in supernatants were measured by using 5 × 105 tumor cells cultured in 24-well plates for 48 hours.
  4. dCells (1 × 103) of each cell line were plated into 60-mm dishes and incubated for 8 days.
  5. eCells (4 × 104) were suspended in 1 ml of 0.3% agar and placed on a presolidified 0.6% agar in 6-well plates and incubated for 3 weeks.
  6. fNot tested.
  7. gNot significant vs implanted FPCK-1-1 cells alone.
  8. *p <0.001, ** p <0.05.
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